Processing math: 100%
Sequence (5’ to 3’) ϵ260nm (M−1cm−1) DOI
CGGGGCGGGCCTTGGGCGGGGT 200400 10.1093/nar/gkt784

       

Structure diagram of 2M27

Structure diagram of 2M27

     

Circular dichroism spectra of the 2M27 oligonucleotide (10 µM), acquired at 25°C in 0.4-cm path-length cuvettes

Circular dichroism spectra of the 2M27 oligonucleotide (10 µM), acquired at 25°C in 0.4-cm path-length cuvettes

 

$^{1}$H-NMR spectrum of the 2M27 oligonucleotide, acquired at 25°C in 100 mM TMAA (pH 7.0) + 1 mM KCl

1H-NMR spectrum of the 2M27 oligonucleotide, acquired at 25°C in 100 mM TMAA (pH 7.0) + 1 mM KCl

 

Folded fraction of the 2M27 oligonucleotide as a function of temperature, determined by UV-melting ($\lambda$ = 295 nm)

Folded fraction of the 2M27 oligonucleotide as a function of temperature, determined by UV-melting (λ = 295 nm)

Native ESI-MS spectra of the 2M27 oligonucleotide (10 µM)

Native ESI-MS spectra of the 2M27 oligonucleotide (10 µM)

 

Native ESI-MS spectra of the 2M27 oligonucleotide (10 µM), focused on the 5$^{-}$ charge state

Native ESI-MS spectra of the 2M27 oligonucleotide (10 µM), focused on the 5− charge state